This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
catalog :
61582
citations: 2
Reference
Chan W, Liu X, Magin R, Girardi N, Ficarro S, Hu W, et al. Accelerating inhibitor discovery for deubiquitinating enzymes. Nat Commun. 2023;14:686 pubmed publisher
Mevissen T, Hospenthal M, Geurink P, Elliott P, Akutsu M, Arnaudo N, et al. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. Cell. 2013;154:169-84 pubmed publisher
product information
Catalog Number :
61582
Product Name :
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
article :
doi10.1016/j.cell.2013.05.046
id7197
pubmed_id23827681
bacterial resistance :
Kanamycin
cloning :
backbonepOPINK
backbone_mutation
backbone_originOPPF-UK
backbone_size6299
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This construct is the Isoform 2 of Cezanne2.
growth strain :
Expresses human Cezanne2 (OTU+UBA domains) in E. coli.
origin :
37
pi :
alt_names
OTUD7A
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5KpnI
promoterT7
sequencing_primer_3T7 Terminal
sequencing_primer_5pGEX 5
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesC15orf16, C16ORF15, CEZANNE2, OTUD7
geneOTUD7A
id161725
genbank_ids
XM_006720413.1
mutationIsoform 2. Deleted aa 463-933.
nameCezanne2
shRNA_sequence
size1386
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis6-GST-3C
resistance markers :
2189
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA