Slc17a7-IRES2-Cre targeting vector | Addgene 61574 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Slc17a7-IRES2-Cre targeting vector

catalog :

61574

citations: 2

Reference
Li M, Tan H, Lu Z, Tsang K, Chung A, Zuker C. Gut-brain circuits for fat preference. Nature. 2022;610:722-730 pubmed publisher
Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L, et al. Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015;85:942-58 pubmed publisher

product information

Catalog Number :

61574

Product Name :

Slc17a7-IRES2-Cre targeting vector

article :

doi	10.1016/j.neuron.2015.02.022
id	10028
pubmed_id	25741722

bacterial resistance :

Ampicillin

cloning :

backbone

minimal cloning vector

backbone_mutation

MCS region was modified

backbone_origin

backbone_size

2657

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mouse Targeting

Cre/Lox

growth notes :

The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.

growth strain :

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TTCACACCGCATAGGGTCAT
sequencing_primer_5	TCCGTCAGGATGGCCTTCTG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Slc17a7-IRES2-Cre

shRNA_sequence

size

15587

species

10090

Mus musculus

32644

Other

P1 Bacteriophage