This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRSETb-pHlash
catalog :
61550
citations: 2
Reference
Zhang Y, Robertson J, Xie Q, Johnson C. Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells. Methods Mol Biol. 2016;1461:117-30 pubmed publisher
Zhang Y, Xie Q, Robertson J, Johnson C. pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH. PLoS ONE. 2012;7:e43072 pubmed publisher
product information
Catalog Number :
61550
Product Name :
pRSETb-pHlash
article :
doi10.1371/journal.pone.0043072
id9400
pubmed_id22905204
bacterial resistance :
Ampicillin
cloning :
backbonepRSETb
backbone_mutation
backbone_originInvitrogen
backbone_size2900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Fusion components were requested from collaborator sources. The complete vector was constructed by the submitting party.
growth strain :
Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification
origin :
37
pi :
alt_names
Rluc8-cpVenus
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterT7
sequencing_primer_3CGCGAATTCTTACTCGATGTTGTGGCGGA
sequencing_primer_5AGGATCCAATGGCTTCCAAGGTGTACGA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namepHlash
shRNA_sequence
size1680
species
100
Synthetic
tags
locationN terminal on backbone
tagHis
resistance markers :
2177
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA