This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCRII_TOPO_hNEAT1
catalog :
61518
citations: 4
Reference
Matsukawa K, Kukharsky M, Park S, Park S, Watanabe N, Iwatsubo T, et al. Long non-coding RNA NEAT1_1 ameliorates TDP-43 toxicity in in vivo models of TDP-43 proteinopathy. RNA Biol. 2021;:1-9 pubmed publisher
Koyama S, Tsuchiya H, Amisaki M, Sakaguchi H, Honjo S, Fujiwara Y, et al. NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44. Int J Mol Sci. 2020;21: pubmed publisher
Cawte A, Unrau P, Rueda D. Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Nat Commun. 2020;11:1283 pubmed publisher
Clemson C, Hutchinson J, Sara S, Ensminger A, Fox A, Chess A, et al. An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles. Mol Cell. 2009;33:717-26 pubmed publisher
product information
Catalog Number :
61518
Product Name :
pCRII_TOPO_hNEAT1
article :
doi10.1016/j.molcel.2009.01.026
id9396
pubmed_id19217333
bacterial resistance :
Ampicillin and Kanamycin
cloning :
backbonePCRII
backbone_mutation
backbone_originLife Technologies
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
general cloning vector
growth notes :
*To create this plasmid, hNEAT1 was amplified from HeLa cDNA. The insert contains two mismatches (C->T at bp 78, and A->G at bp 91, an A->G at bp 1666, and a deletion of TA at bp 2031 and 2032 using the numbering of NR_028272.1) compared to the canonical hNEAT1 sequence. These variants are likely to be encoded in the HeLa genome, as they appeared in multiple clones, but this is yet to be verified by sequencing of HeLa genomic DNA.
growth strain :
human NEAT1_v1 short isoform 3.7kb insert in general cloning vector
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CAGGAAACAGCTATGAC
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLINC00084, NCRNA00084, TP53LC15, TncRNA, VINC
geneNEAT1
id283131
genbank_ids
NR_028272.1
mutation*see comment below
nameNEAT1
shRNA_sequence
size3700
species
9606
Homo sapiens
tags
resistance markers :
2174
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA