This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RAB14 3'UTR D2
catalog :
61491
citations: 1
Reference
Kim M, Tan Y, Cheng W, Kingsbury T, Heimfeld S, Civin C. MIR144 and MIR451 regulate human erythropoiesis via RAB14. Br J Haematol. 2015;168:583-97 pubmed publisher
product information
Catalog Number :
61491
Product Name :
RAB14 3'UTR D2
article :
doi10.1111/bjh.13164
id9246
pubmed_id25312678
bacterial resistance :
Ampicillin
cloning :
backbonepmirGLO Dual-Luciferase
backbone_mutation
backbone_originPromega
backbone_size7350
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Oligos used for site-directed mutagenesis: F-CAGGTGGTTTGGCCACTGGCAAGAGAAACAATCTTA R-TAAGATTGTTTCTCTTGCCAGTGGCCAAACCACCTG These 10bp deletion constructs were used in BJH (2015).
growth strain :
luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
origin :
37
pi :
alt_names
RAB14
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NheI
promoterPGK
sequencing_primer_3
sequencing_primer_5Luc-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFBP, RAB-14
geneRAB14
id51552
genbank_ids
NM_016322
mutationcontains position 837-1637 (numbering relative to start of 3'utr) and second predicted MIR144 binding site deleted, 10bp deletion
nameRAB14 3' UTR
shRNA_sequence
size801
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagluciferase
resistance markers :
2172
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA