FUGW-PK-hLC3 G | Addgene 61461 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

FUGW-PK-hLC3 G

catalog :

61461

citations: 4

Reference
Xiong J, Luu T, Venkatachalam K, Du G, Zhu M. Glutamine Produces Ammonium to Tune Lysosomal pH and Regulate Lysosomal Function. Cells. 2022;12: pubmed publisher
Beesabathuni N, Park S, Shah P. Quantitative and temporal measurement of dynamic autophagy rates. Autophagy. 2022;:1-20 pubmed publisher
Tanida I, Ueno T, Uchiyama Y. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses. Methods Enzymol. 2017;587:87-96 pubmed publisher
Tanida I, Ueno T, Uchiyama Y. A super-ecliptic, pHluorin-mKate2, tandem fluorescent protein-tagged human LC3 for the monitoring of mammalian autophagy. PLoS ONE. 2014;9:e110600 pubmed publisher

product information

Catalog Number :

61461

Product Name :

FUGW-PK-hLC3 G

article :

doi	10.1371/journal.pone.0110600
id	9276
pubmed_id	25340751

bacterial resistance :

Ampicillin

cloning :

backbone

FUGW

backbone_mutation

backbone_origin

backbone_size

9000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

We thank Dr. James Edward Rothman for providing respective pHluorin.

growth strain :

Express pHluorin-mKate2-hLC3 G (PK-hLC3 G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

origin :

pi :

alt_names

pHluorin-mKate2-hLC3 G

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	hUBC
sequencing_primer_3
sequencing_primer_5	TGTGGACAGAAGACTGGAAAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
gene	MAP1LC3B
id	81631

genbank_ids

mutation

name

PK-hLC3 G

shRNA_sequence

size

species

9606

Homo sapiens

32644

Other