This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FUGW-PK-hLC3
catalog :
61460
citations: 11
Reference
Li Y, Liu Y, Yao X, Zhu L, Yang L, Zhan Q. MLKL Protects Pulmonary Endothelial Cells in Acute Lung Injury. Am J Respir Cell Mol Biol. 2024;: pubmed publisher
Beesabathuni N, Park S, Shah P. Quantitative and temporal measurement of dynamic autophagy rates. Autophagy. 2022;:1-20 pubmed publisher
Magalhães Novais S, Blecha J, Naraine R, Mikešová J, Abaffy P, Pecinova A, et al. Mitochondrial respiration supports autophagy to provide stress resistance during quiescence. Autophagy. 2022;:1-18 pubmed publisher
Zhan Q, Jeon J, Li Y, Huang Y, Xiong J, Wang Q, et al. CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux. Autophagy. 2021;:1-19 pubmed publisher
Shen H, Li J, Heisler Taylor T, Makin R, Yang H, Mavlyutov T, et al. TMEM97 ablation aggravates oxidant-induced retinal degeneration. Cell Signal. 2021;86:110078 pubmed publisher
Santerre M, Arjona S, Allen C, Callen S, Buch S, Sawaya B. HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. Autophagy. 2021;17:1768-1782 pubmed publisher
Huang X, Huang J, Du J, Zhang N, Long Z, Yang Y, et al. Autophagy inhibitors increase the susceptibility of KRAS-mutant human colorectal cancer cells to a combined treatment of 2-deoxy-D-glucose and lovastatin. Acta Pharmacol Sin. 2021;: pubmed publisher
Minami S, Shah P. Transient light-activated gene expression in Chinese hamster ovary cells. BMC Biotechnol. 2021;21:13 pubmed publisher
Shang C, Hassan B, Haque M, Song Y, Li J, Liu D, et al. Crizotinib Resistance Mediated by Autophagy Is Higher in the Stem-Like Cell Subset in ALK-Positive Anaplastic Large Cell Lymphoma, and This Effect Is MYC-Dependent. Cancers (Basel). 2021;13: pubmed publisher
Tanida I, Ueno T, Uchiyama Y. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses. Methods Enzymol. 2017;587:87-96 pubmed publisher
Tanida I, Ueno T, Uchiyama Y. A super-ecliptic, pHluorin-mKate2, tandem fluorescent protein-tagged human LC3 for the monitoring of mammalian autophagy. PLoS ONE. 2014;9:e110600 pubmed publisher
product information
Catalog Number :
61460
Product Name :
FUGW-PK-hLC3
article :
doi10.1371/journal.pone.0110600
id9276
pubmed_id25340751
bacterial resistance :
Ampicillin
cloning :
backboneFUGW
backbone_mutation
backbone_origin
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
We thank Dr. James Edward Rothman for providing respective pHluorin.
growth strain :
Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)
origin :
37
pi :
alt_names
pHluorin-mKate2-hLC3
cloning
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
mutation
namePK-hLC3
shRNA_sequence
size
species
9606
Homo sapiens
32644
Other
tags
resistance markers :
739
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA