This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEX-PK-hLC3
catalog :
61458
citations: 3
Reference
Tanida I, Kakuta S, Oliva Trejo J, Uchiyama Y. Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein. Sci Rep. 2020;10:11314 pubmed publisher
Tanida I, Ueno T, Uchiyama Y. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses. Methods Enzymol. 2017;587:87-96 pubmed publisher
Tanida I, Ueno T, Uchiyama Y. A super-ecliptic, pHluorin-mKate2, tandem fluorescent protein-tagged human LC3 for the monitoring of mammalian autophagy. PLoS ONE. 2014;9:e110600 pubmed publisher
product information
Catalog Number :
61458
Product Name :
pEX-PK-hLC3
article :
doi10.1371/journal.pone.0110600
id9276
pubmed_id25340751
bacterial resistance :
Ampicillin
cloning :
backbonepCXN2/ pCAGGS
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
We thank Dr. James Edward Rothman for providing respective pHluorin.
growth strain :
Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy
origin :
37
pi :
alt_names
pHluorin-mKate2-hLC3
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterCAG
sequencing_primer_3AGCCACCACCTTCTGATAG
sequencing_primer_5GGCTTCTGGCGTGTGACC
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
mutation
namePK-hLC3
shRNA_sequence
size
species
9606
Homo sapiens
4896
Schizosaccharomyces pombe
32644
Other
tags
resistance markers :
739
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA