This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MS2-P65-HSF1_GFP
catalog :
61423
citations: 39
Reference
Su Tobon Q, Fan J, Feeney K, Ren H, Autissier P, Wang P, et al. CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers. bioRxiv. 2023;: pubmed publisher
Sgro A, Cursons J, Waryah C, Woodward E, Foroutan M, Lyu R, et al. Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma. Clin Epigenetics. 2023;15:73 pubmed publisher
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Chen W, Gaidukov L, Lai Y, Wu M, Cao J, Gutbrod M, et al. A synthetic transcription platform for programmable gene expression in mammalian cells. Nat Commun. 2022;13:6167 pubmed publisher
Chan W, Coughlan H, Chen Y, Keenan C, Smyth G, Perkins A, et al. Activation of stably silenced genes by recruitment of a synthetic de-methylating module. Nat Commun. 2022;13:5582 pubmed publisher
Leung H, Foo G, VanDongen A. Arc Regulates Transcription of Genes for Plasticity, Excitability and Alzheimer's Disease. Biomedicines. 2022;10: pubmed publisher
Wang J, Wang A, Tian K, Hua X, Zhang B, Zheng Y, et al. A Ctnnb1 enhancer regulates neocortical neurogenesis by controlling the abundance of intermediate progenitors. Cell Discov. 2022;8:74 pubmed publisher
Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, et al. Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Nucleic Acids Res. 2022;50:7842-7855 pubmed publisher
Ma H, de Zwaan E, Guo Y, Cejas P, Thiru P, van de Bunt M, et al. The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes. Cell Stem Cell. 2022;29:795-809.e11 pubmed publisher
Wang C, Qu Y, Cheng J, Hughes N, Zhang Q, Wang M, et al. dCas9-based gene editing for cleavage-free genomic knock-in of long sequences. Nat Cell Biol. 2022;24:268-278 pubmed publisher
Fennell K, Vassiliadis D, Lam E, Martelotto L, Balic J, Hollizeck S, et al. Non-genetic determinants of malignant clonal fitness at single-cell resolution. Nature. 2022;601:125-131 pubmed publisher
Wang C, Xia Q, Zhang Q, Qu Y, Su S, Cheng J, et al. CRISPR-Cas12a System With Synergistic Phage Recombination Proteins for Multiplex Precision Editing in Human Cells. Front Cell Dev Biol. 2021;9:719705 pubmed publisher
Zeng J, Xu H, Huang C, Sun Y, Xiao H, Yu G, et al. CD46 splice variant enhances translation of specific mRNAs linked to an aggressive tumor cell phenotype in bladder cancer. Mol Ther Nucleic Acids. 2021;24:140-153 pubmed publisher
Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, et al. MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021;49:4171-4185 pubmed publisher
Lu X, Chen X, Forney C, Donmez O, Miller D, Parameswaran S, et al. Global discovery of lupus genetic risk variant allelic enhancer activity. Nat Commun. 2021;12:1611 pubmed publisher
Wang C, Cheng J, Zhang Q, Hughes N, Xia Q, Winslow M, et al. Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects. Nucleic Acids Res. 2021;49:e36 pubmed publisher
Hamann M, Ehmele P, Verdikt R, Bialek Waldmann J, Virdi S, Gunther T, et al. Transcriptional behavior of the HIV-1 promoter in context of the BACH2 prominent proviral integration gene. Virus Res. 2021;293:198260 pubmed publisher
Velazquez J, LeGraw R, Moghadam F, Tan Y, Kilbourne J, Maggiore J, et al. Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Cell Syst. 2021;12:41-55.e11 pubmed publisher
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Ferry Q, Fulga T. Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs. Methods Mol Biol. 2021;2162:153-184 pubmed publisher
Hsu M, Liao H, Truong V, Huang K, Yu F, Chen H, et al. CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Theranostics. 2019;9:6099-6111 pubmed publisher
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Yamazaki T, Fujikawa C, Kubota A, Takahashi A, Hirose T. CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles. Biochem Biophys Res Commun. 2018;504:218-224 pubmed publisher
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Ferry Q, Lyutova R, Fulga T. Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Nat Commun. 2017;8:14633 pubmed publisher
Hu L, Yu Y, Huang H, Fan H, Hu L, Yin C, et al. Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis. Front Immunol. 2016;7:696 pubmed publisher
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product information
Catalog Number :
61423
Product Name :
MS2-P65-HSF1_GFP
article :
doi10.1038/nature14136
id9503
pubmed_id25494202
bacterial resistance :
Ampicillin
cloning :
backbonelenti(AMP)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
For additional information, protocols and an activator sgRNA design tool, visit our website: http://sam.genome-engineering.org/
growth strain :
Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BsiWI
promoterEF1A
sequencing_primer_3cacatagcgtaaaaggagcaacatag
sequencing_primer_5GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationN55K in MS2
nameMS2(N55K)-P65-HSF1_2A_GFP
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
resistance markers :
898
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA