This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOPINK-OTUB1 (full-length, aa 1-271)
catalog :
61420
citations: 2
Reference
Kniss A, Schuetz D, Kazemi S, Pluska L, Spindler P, Rogov V, et al. Chain Assembly and Disassembly Processes Differently Affect the Conformational Space of Ubiquitin Chains. Structure. 2018;26:249-258.e4 pubmed publisher
Mevissen T, Hospenthal M, Geurink P, Elliott P, Akutsu M, Arnaudo N, et al. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. Cell. 2013;154:169-84 pubmed publisher
product information
Catalog Number :
61420
Product Name :
pOPINK-OTUB1 (full-length, aa 1-271)
article :
doi10.1016/j.cell.2013.05.046
id7197
pubmed_id23827681
bacterial resistance :
Kanamycin
cloning :
backbonepOPINK
backbone_mutation
backbone_originOPPF-UK
backbone_size6299
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
growth strain :
Expresses human OTUB1 (full-length) in E. coli.
origin :
37
pi :
alt_names
Otubain-1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5KpnI
promoterT7
sequencing_primer_3T7 Terminal
sequencing_primer_5pGEX 5
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesHSPC263, OTB1, OTU1
geneOTUB1
id55611
genbank_ids
NM_017670.2
mutation
nameOTUB1 (Ubiquitin thioesterase OTUB1)
shRNA_sequence
size813
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis6-GST-3C
resistance markers :
2189
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA