pBTR(hCc) | Addgene 61026 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBTR(hCc)

catalog :

61026

citations: 2

Reference
Li J, Li H. New insights into the folding-unfolding mechanism and conformations of cytochrome C. Chem Sci. 2022;13:7498-7508 pubmed publisher
Patel C, Lind M, Pielak G. Characterization of horse cytochrome c expressed in Escherichia coli. Protein Expr Purif. 2001;22:220-4 pubmed

product information

Catalog Number :

61026

Product Name :

pBTR(hCc)

article :

doi	10.1006/prep.2001.1438
id	8515
pubmed_id	11437597

bacterial resistance :

Ampicillin

cloning :

backbone

pBTR(C102T)

backbone_mutation

backbone_origin

Multiple labs

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Notes from depositor: CYC3 encodes the enzyme, which we call heme lysase in the paper, that covalently attaches the heme to the apocytochrome c. That is, the plasmid has BOTH the CYC3 gene and the structural gene for horse cytochrome c. The horse cytochrome C sequence has been codon optimized.

growth strain :

Expresses horse holocytochrome c in E. coli

growth temp :

Depositing laboratory recommends using bacterial strain BL21(DE3) for protein expression

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	NcoI
promoter
sequencing_primer_3	M13 (-21) Forward
sequencing_primer_5	M13 Reverse
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	CYCS
id	100053958

genbank_ids

NP_001157486.1

mutation

*see comments

name

horse cytochrome c

shRNA_sequence

size

species

32644

Other

Equus*