This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPL5618_pUG_MtxR
catalog :
60929
citations: 1
Reference
MacDonald C, Piper R. Puromycin- and methotrexate-resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast. Yeast. 2015;32:423-38 pubmed publisher
product information
Catalog Number :
60929
Product Name :
pPL5618_pUG_MtxR
article :
doi10.1002/yea.3069
id9780
pubmed_id25688547
bacterial resistance :
Ampicillin
cloning :
backbonepUG27
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
LoxP flanked deletion cassette template
growth notes :
Dihydrofolate reductase gene from Homo sapiens carrying L22F and F31S mutations (DHFR*); codon optimized for expression in Saccharomyces cerevisiae. It also contains an additional F180L mutation that does not impact protein function.
growth strain :
PCR template vector for generating methotrexate resistance cassette.
origin :
37
pi :
alt_names
DHFR
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTEF1
sequencing_primer_3gggcagatgatgtcgaggcg
sequencing_primer_5cctcgctgcagacctgcgagcagg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDHFR1, DHFRP1, DYR
geneDHFR
id1719
genbank_ids
mutationL22F F31S
nameDihydrofolate reductase
shRNA_sequence
size561
species
9606
Homo sapiens
tags
plasmid copy :
They were gene synthesized using a commercial service, which made them according to our design.
resistance markers :
2139
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA