This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTight-9-124-BclxL
catalog :
60857
citations: 9
Reference
Zhu B, Fisher E, Li L, Zhong P, Yan Z, Feng J. PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes. Stem Cell Reports. 2023;18:2268-2282 pubmed publisher
Yoo A, Lee S, Oh Y, VICTOR M, Strunilin I, Chen S, et al. Longitudinal modeling of human neuronal aging identifies RCAN1-TFEB pathway contributing to neurodegeneration of Huntington's disease. Res Sq. 2023;: pubmed publisher
Kraskovskaya N, Bolshakova A, Khotin M, Bezprozvanny I, Mikhailova N. Protocol Optimization for Direct Reprogramming of Primary Human Fibroblast into Induced Striatal Neurons. Int J Mol Sci. 2023;24: pubmed publisher
Church V, Cates K, Capano L, Aryal S, Kim W, Yoo A. Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts. Methods Mol Biol. 2021;2239:77-100 pubmed publisher
Lee S, Oh Y, Lü Y, Kim W, Yoo A. MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts. Dev Cell. 2018;46:73-84.e7 pubmed publisher
Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
Gaj T, Staahl B, Rodrigues G, Limsirichai P, Ekman F, Doudna J, et al. Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Nucleic Acids Res. 2017;45:e98 pubmed publisher
Richner M, Victor M, Liu Y, Abernathy D, Yoo A. MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons. Nat Protoc. 2015;10:1543-55 pubmed publisher
Victor M, Richner M, Hermanstyne T, Ransdell J, Sobieski C, Deng P, et al. Generation of human striatal neurons by microRNA-dependent direct conversion of fibroblasts. Neuron. 2014;84:311-23 pubmed publisher
product information
Catalog Number :
60857
Product Name :
pTight-9-124-BclxL
article :
doi10.1016/j.neuron.2014.10.016
id9463
pubmed_id25374357
bacterial resistance :
Ampicillin
cloning :
backbonepTLemiR
backbone_mutation
backbone_originHomemade
backbone_size10335
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
origin :
37
pi :
alt_names
microRNA 9, microRNA 124, BCL2-like 1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterpTight
sequencing_primer_3GCACACCGGCCTTATTCCAAG
sequencing_primer_5AGCGCTGCCACCATGTCTCA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52
geneBCL2L1
id598
genbank_ids
NR_029818 NR_029814
NM_138578.1
mutation
namemiR-9/9*-miR-124-BCLxL
shRNA_sequence
size1371
species
10090
Mus musculus
tags
resistance markers :
2131
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA