This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
NaChBac pTracer CMV2
catalog :
60835
citations: 2
Reference
Helliwell K, Chrachri A, Koester J, Wharam S, Verret F, Taylor A, et al. Alternative Mechanisms for Fast Na+/Ca2+ Signaling in Eukaryotes via a Novel Class of Single-Domain Voltage-Gated Channels. Curr Biol. 2019;29:1503-1511.e6 pubmed publisher
Ren D, Navarro B, Xu H, Yue L, Shi Q, Clapham D. A prokaryotic voltage-gated sodium channel. Science. 2001;294:2372-5 pubmed
product information
Catalog Number :
60835
Product Name :
NaChBac pTracer CMV2
article :
doi10.1126/science.1065635
id9207
pubmed_id11743207
bacterial resistance :
Ampicillin
cloning :
backboneModified pTracer -CMV2
backbone_mutation
backbone_origin
backbone_size6210
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
NOTE: The EGFP in this plasmid does has some discrepancies with the EGFP reported in the standard pTracer -CMV2. These modifications do not appear to change the function or effectivity of the EGFP.
growth strain :
Mammalian expression plasmid containing NaChBac gene with an EGFP reporter
origin :
37
pi :
alt_names
NavBh
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoR1
promoterCMV
sequencing_primer_3CACGCCTACCGCCCATTTGCG
sequencing_primer_5CGC AAA TGG GCG GTA GGC GTG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
NP_242367.1
mutation
nameNaChBac
shRNA_sequence
size825
species
32644
Other
Bacillus Hallodurans C-125
tags
resistance markers :
2125
tags :
High Copy
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA