This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRevLCav2deltaC
catalog :
60809
citations: 1
Reference
Siebler H, Lada A, Baranovskiy A, Tahirov T, Pavlov Y. A novel variant of DNA polymerase ?, Rev3?C, highlights differential regulation of Pol32 as a subunit of polymerase ? versus ? in Saccharomyces cerevisiae. DNA Repair (Amst). 2014;24:138-149 pubmed publisher
product information
Catalog Number :
60809
Product Name :
pRevLCav2deltaC
article :
doi10.1016/j.dnarep.2014.04.013
id9193
pubmed_id24819597
bacterial resistance :
Ampicillin
cloning :
backbonepYIY118
backbone_mutationURA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
backbone_originthis lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Artem Lada in the Pavlov lab and Tahir Tahirov (UNMC) contributed to the creation of this construct.
growth strain :
to make truncation of yeast Rev3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoter
sequencing_primer_3M13R
sequencing_primer_5M13-F20
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL167C, PSO1
geneREV3
id855936
genbank_ids
mutationrev3 C encodes for Rev3 lacking the C-terminus (amino acids 1381 1504)
nameREV3
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
2124
2126
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA