pRevLCav2FeS | Addgene 60808 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRevLCav2FeS

catalog :

60808

citations: 1

Reference
Baranovskiy A, Lada A, Siebler H, Zhang Y, Pavlov Y, Tahirov T. DNA polymerase ? and ? switch by sharing accessory subunits of DNA polymerase ?. J Biol Chem. 2012;287:17281-7 pubmed publisher

product information

Catalog Number :

60808

Product Name :

pRevLCav2FeS

article :

doi	10.1074/jbc.M112.351122
id	9192
pubmed_id	22465957

bacterial resistance :

Ampicillin

cloning :

backbone

pYIY118

backbone_mutation

URA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.

backbone_origin

this lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Artem Lada in the Pavlov lab and Tahir Tahirov (UNMC) contributed to the creation of this construct.

growth strain :

to disrupt iron sulfur cluster in yeast Rev3

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	SmaI
promoter
sequencing_primer_3	M13R
sequencing_primer_5	M13-F20
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	YPL167C, PSO1
gene	REV3
id	855936

genbank_ids

mutation

contains 647 C-terminal amino acids and 335 bp of downstream noncoding sequence, change of cysteines 1446, 1449, 1468, and 1473 at MBS2 to alanines

name

REV3

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae