This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYIAL31
catalog :
60807
citations: 1
Reference
Pavlov Y, Shcherbakova P, Kunkel T. In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta. Genetics. 2001;159:47-64 pubmed
product information
Catalog Number :
60807
Product Name :
pYIAL31
article :
doi
id9191
pubmed_id11560886
bacterial resistance :
Ampicillin
cloning :
backbonepYIY118
backbone_mutationURA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
backbone_originthis lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
used to make mutations in yeast POL1
origin :
37
pi :
alt_names
DNA-directed DNA polymerase alpha catalytic subunit POL1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5M13-F20
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYNL102W, CDC17, CRT5, HPR3
genePOL1
id855621
genbank_ids
mutationN-terminal part only
namePol1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
2123
2124
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA