This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIS1 LMBRD2
catalog :
60800
citations: 1
Reference
Nam J, Rissland O, Koppstein D, Abreu Goodger C, Jan C, Agarwal V, et al. Global analyses of the effect of different cellular contexts on microRNA targeting. Mol Cell. 2014;53:1031-1043 pubmed publisher
product information
Catalog Number :
60800
Product Name :
pIS1 LMBRD2
article :
doi10.1016/j.molcel.2014.02.013
id9190
pubmed_id24631284
bacterial resistance :
Ampicillin
cloning :
backbonepIS1
backbone_mutation
backbone_originBartel lab (Addgene plasmid # 12179)
backbone_size4085
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and wild-type miR-155 sites
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XbaI
promoterthymidine kinase (HSV-TK promoter)
sequencing_primer_3EBV-rev
sequencing_primer_5chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3') to confirm luciferase
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneLMBRD2
id92255
genbank_ids
mutation
nameLMBRD2 3'UTR and wild-type miR-155 binding site
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA