This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIS1 mEPAS1
catalog :
60793
citations: 1
Reference
Nam J, Rissland O, Koppstein D, Abreu Goodger C, Jan C, Agarwal V, et al. Global analyses of the effect of different cellular contexts on microRNA targeting. Mol Cell. 2014;53:1031-1043 pubmed publisher
product information
Catalog Number :
60793
Product Name :
pIS1 mEPAS1
article :
doi10.1016/j.molcel.2014.02.013
id9190
pubmed_id24631284
bacterial resistance :
Ampicillin
cloning :
backbonepIS1
backbone_mutation
backbone_originBartel lab (Addgene plasmid # 12179)
backbone_size4085
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XbaI
promoterthymidine kinase (HSV-TK promoter)
sequencing_primer_3EBV-rev
sequencing_primer_5chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3') to confirm luciferase
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
geneEPAS1
id2034
genbank_ids
mutationmutated miR-155 binding site
nameEPAS1 3'UTR and mutated miR-155 binding site
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA