This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SpDJ-1 pET15b
catalog :
60689
citations: 1
Reference
Madzelan P, Labunska T, Wilson M. Influence of peptide dipoles and hydrogen bonds on reactive cysteine pKa values in fission yeast DJ-1. FEBS J. 2012;279:4111-20 pubmed publisher
product information
Catalog Number :
60689
Product Name :
SpDJ-1 pET15b
article :
doi10.1111/febs.12004
id9171
pubmed_id22971103
bacterial resistance :
Ampicillin
cloning :
backbonepET15b
backbone_mutation
backbone_originNovagen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Sequence is synthetic with codon usage optimized for E.coli expression.
growth strain :
Recombinant protein expression of DJ-1
origin :
37
pi :
alt_names
SPAC22E12.03c
(See Comments)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPAC22E12.03c
geneSPAC22E12.03c
id2541772
genbank_ids
mutation
nameDJ-1
shRNA_sequence
size
species
4896
Schizosaccharomyces pombe
tags
locationN terminal on backbone
tag6xHis
resistance markers :
2111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA