DJ-1 TEV site pET15b | Addgene 60687 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

DJ-1 TEV site pET15b

catalog :

60687

citations: 3

Reference
Jimenez Harrison D, Huseby C, Hoffman C, Sher S, Snyder D, Seal B, et al. DJ-1 Molecular Chaperone Activity Depresses Tau Aggregation Propensity through Interaction with Monomers. Biochemistry. 2023;62:976-988 pubmed publisher
Barbieri L, Luchinat E. Backbone resonance assignment of human DJ-1 in the reduced state and in the cysteine sulfinic acid state. Biomol NMR Assign. 2019;13:371-376 pubmed publisher
Barbieri L, Luchinat E, Banci L. Intracellular metal binding and redox behavior of human DJ-1. J Biol Inorg Chem. 2018;23:61-69 pubmed publisher

product information

Catalog Number :

60687

Product Name :

DJ-1 TEV site pET15b

article :

doi
id	9170
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pET15b

backbone_mutation

backbone_origin

Novagen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This construct contains a Tobacco etch virus (TEV) protease recognition sequence (ENLYFQS) after the Thromin recognition site, and just before DJ-1. (Ex. LVPRGSHMENLYFQSASKRAL. )-TEV site italicized. Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T).

growth strain :

Recombinant protein expression of Park7

origin :

pi :

alt_names

PARK 7

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	T7
sequencing_primer_3	T7term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DJ-1, DJ1, GATD2, HEL-S-67p
gene	PARK7
id	11315

genbank_ids

mutation

TEV site was inserted (see comments below).

name

DJ-1

shRNA_sequence

size

species

9606

Homo sapiens