This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M26L DJ-1 TEV site pET15b
catalog :
60686
product information
Catalog Number :
60686
Product Name :
M26L DJ-1 TEV site pET15b
article :
doi
id9170
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepET15b
backbone_mutation
backbone_originNovagen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This construct contains a Tobacco etch virus (TEV) protease recognition sequence (ENLYFQS) after the inactivated Thrombin recognition site (mutation of Leu (L) to Gln (Q), underlined below), and just before DJ-1. (Ex. QVPRGSHMENLYFQSASKRAL. ). The TEV site is italicized and the thrombin cleavage site is NOT intact for the M26L mutant of DJ-1. Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T).
growth strain :
Recombinant protein expression of Park7
origin :
37
pi :
alt_names
PARK 7
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDJ-1, DJ1, GATD2, HEL-S-67p
genePARK7
id11315
genbank_ids
mutationM26L and TEV site was inserted (see comments below).
nameM26L DJ-1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis
resistance markers :
2111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA