This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA 3.1 (+) FLAG-Ago2 S387E
catalog :
60653
citations: 1
Reference
Horman S, Janas M, Litterst C, Wang B, Macrae I, Sever M, et al. Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013;50:356-67 pubmed publisher
product information
Catalog Number :
60653
Product Name :
pcDNA 3.1 (+) FLAG-Ago2 S387E
article :
doi10.1016/j.molcel.2013.03.015
id9004
pubmed_id23603119
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation. +/- refers to orientation of f1 ori.
growth strain :
Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells
origin :
37
pi :
alt_names
Protein Slicer
EIF2C 2
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
mutationS387E mutant
nameArgonaute 2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagCMV-FLAG-Ago2 (N-term tag)
resistance markers :
366
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA