This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+) CircRNA Mini Vector
catalog :
60648
citations: 26
Reference
Fang X, Ao X, Xiao D, Wang Y, Jia Y, Wang P, et al. Circular RNA-circPan3 attenuates cardiac hypertrophy via miR-320-3p/HSP20 axis. Cell Mol Biol Lett. 2024;29:3 pubmed publisher
Unti M, Jaffrey S. Highly efficient cellular expression of circular mRNA enables prolonged protein expression. bioRxiv. 2023;: pubmed publisher
Peng W, Ye L, Xue Q, Wei X, Wang Z, Xiang X, et al. Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion. Cells. 2023;12: pubmed publisher
Gosavi D, Wower I, Beckmann I, Hofacker I, Wower J, Wolfinger M, et al. Insights into the secondary and tertiary structure of the Bovine Viral Diarrhea Virus Internal Ribosome Entry Site. RNA Biol. 2022;19:496-506 pubmed publisher
Zhu Y, Li J, Liu H, Song Z, Yang Q, Lu C, et al. Circular RNA, hsa_circRNA_102049, promotes colorectal cancer cell migration and invasion via binding and suppressing miRNA-455-3p. Exp Ther Med. 2022;23:244 pubmed publisher
Siqueira E, Obiols Guardia A, Jorge Torres O, Oliveira Mateos C, Soler M, Ramesh Kumar D, et al. Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome. Mol Ther Nucleic Acids. 2022;27:621-644 pubmed publisher
Wong C, Lou U, Fung F, Tong J, Zhang C, To K, et al. CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein. Mol Cancer. 2022;21:10 pubmed publisher
Mei X, Cui X, Li Y, Chen S. CircSOD2: A Novel Regulator for Smooth Muscle Proliferation and Neointima Formation. Arterioscler Thromb Vasc Biol. 2021;41:2961-2973 pubmed publisher
Hong X, Li S, Wang J, Zhao Z, Feng Z. Circular RNA circFADS2 is overexpressed in sepsis and suppresses LPS-induced lung cell apoptosis by inhibiting the maturation of miR-15a-5p. BMC Immunol. 2021;22:29 pubmed publisher
Zhang H, Xiao X, Wei W, Huang C, Wang M, Wang L, et al. CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer. Mol Cancer. 2021;20:70 pubmed publisher
Karedath T, Al Dasim F, Ahmed I, Al Qurashi A, Raza A, Andrews S, et al. Regulation of Circular RNA CircNFATC3 in Cancer Cells Alters Proliferation, Migration, and Oxidative Phosphorylation. Front Cell Dev Biol. 2021;9:595156 pubmed publisher
Zhang Y, Hong Y, Wang D, Duan L, Liu Y, Li L, et al. Hsa_circ_0076305 induces migration-proliferation dichotomy in gastric cancer. Oncol Lett. 2021;21:220 pubmed publisher
Lou J, Hao Y, Lin K, Lyu Y, Chen M, Wang H, et al. Circular RNA CDR1as disrupts the p53/MDM2 complex to inhibit Gliomagenesis. Mol Cancer. 2020;19:138 pubmed publisher
Lv T, Miao Y, Xu T, Sun W, Sang Y, Jia F, et al. Circ-EPB41L5 regulates the host gene EPB41L5 via sponging miR-19a to repress glioblastoma tumorigenesis. Aging (Albany NY). 2020;12:318-339 pubmed publisher
Wang F, Chen X, Han Y, Xi S, Wu G. circRNA CDR1as Regulated the Proliferation of Human Periodontal Ligament Stem Cells under a Lipopolysaccharide-Induced Inflammatory Condition. Mediators Inflamm. 2019;2019:1625381 pubmed publisher
Chen S, Huang V, Xu X, Livingstone J, Soares F, Jeon J, et al. Widespread and Functional RNA Circularization in Localized Prostate Cancer. Cell. 2019;176:831-843.e22 pubmed publisher
Wang Z, Ma K, Pitts S, Cheng Y, Liu X, Ke X, et al. Novel circular RNA NF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16. Endocr Relat Cancer. 2018;: pubmed publisher
Liu T, Song Z, Gai Y. Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p. Biochem Biophys Res Commun. 2018;503:1503-1509 pubmed publisher
Qi Y, Zhang B, Wang J, Yao M. Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo. Gene. 2018;676:79-85 pubmed publisher
Wang Y, Sui X, Zhao H, Cong L, Li Y, Xin T, et al. Decreased circular RNA hsa_circ_0001649 predicts unfavorable prognosis in glioma and exerts oncogenic properties in vitro and in vivo. Gene. 2018;676:117-122 pubmed publisher
Xing L, Zhang L, Feng Y, Cui Z, Ding L. Downregulation of circular RNA hsa_circ_0001649 indicates poor prognosis for retinoblastoma and regulates cell proliferation and apoptosis via AKT/mTOR signaling pathway. Biomed Pharmacother. 2018;105:326-333 pubmed publisher
Zhang X, Luo P, Jing W, Zhou H, Liang C, Tu J. circSMAD2 inhibits the epithelial-mesenchymal transition by targeting miR-629 in hepatocellular carcinoma. Onco Targets Ther. 2018;11:2853-2863 pubmed publisher
Zhang X, Zhou H, Jing W, Luo P, Qiu S, Liu X, et al. The Circular RNA hsa_circ_0001445 Regulates the Proliferation and Migration of Hepatocellular Carcinoma and May Serve as a Diagnostic Biomarker. Dis Markers. 2018;2018:3073467 pubmed publisher
Zhang X, Qiu S, Luo P, Zhou H, Jing W, Liang C, et al. Down-regulation of hsa_circ_0001649 in hepatocellular carcinoma predicts a poor prognosis. Cancer Biomark. 2018;22:135-142 pubmed publisher
Xu Z, Yang M, Liu H, Su C. Circular RNA hsa_circ_0003221 (circPTK2) promotes the proliferation and migration of bladder cancer cells. J Cell Biochem. 2018;119:3317-3325 pubmed publisher
Liang D, Wilusz J. Short intronic repeat sequences facilitate circular RNA production. Genes Dev. 2014;28:2233-47 pubmed publisher
product information
Catalog Number :
60648
Product Name :
pcDNA3.1(+) CircRNA Mini Vector
article :
doi10.1101/gad.251926.114
id9189
pubmed_id25281217
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originLife Technologies
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A 51-nt artificial exon composed of restriction enzyme sites was inserted between the minimal introns that support ZKSCAN1 circular RNA production (as described in Figure 7A of Liang and Wilusz (2014) Genes Dev).
growth strain :
Expression plasmid for expressing circular RNAs of a desired sequence
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3bGH PolyA Rev
sequencing_primer_5PCMV For
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKOX18, PHZ-37, ZNF139, ZNF36, ZSCAN33
geneZKSCAN1
id7586
genbank_ids
mutation
nameZKSCAN1
shRNA_sequence
size199
species
9606
Homo sapiens
tags
resistance markers :
2108
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA