This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMXs-puro-RFP-ATG9A
catalog :
60609
citations: 5
Reference
Broadbent D, Barnaba C, Perez G, Schmidt J. Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formation. J Cell Biol. 2023;222: pubmed publisher
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P. Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates. Nat Commun. 2023;14:455 pubmed publisher
Chen F, Amgalan D, Kitsis R, Pessin J, Feng D. ATG16L1 autophagy pathway regulates BAX protein levels and programmed cell death. J Biol Chem. 2020;295:15045-15053 pubmed publisher
Stavoe A, Gopal P, Gubas A, Tooze S, Holzbaur E. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. elife. 2019;8: pubmed publisher
Koyama Honda I, Itakura E, Fujiwara T, Mizushima N. Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy. 2013;9:1491-9 pubmed publisher
product information
Catalog Number :
60609
Product Name :
pMXs-puro-RFP-ATG9A
article :
doi10.4161/auto.25529
id8739
pubmed_id23884233
bacterial resistance :
Ampicillin
cloning :
backbonepMXs-puro
backbone_mutation
backbone_originDr.Toshio Kitamura of the University of Tokyo
backbone_size6878
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The S592G and H669N mutations found in Addgene's QC results are natural variants (SNP)
growth strain :
Expresses RFP-hATG9A in mammalian cells
origin :
37
pi :
alt_names
ATG9
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3gggaattcTCTATACCTTGTGCACCTGAGG
sequencing_primer_5gggaattccATGGCGCAGTTTGACACTGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
genePPARG
id5468
genbank_ids
NM024085
mutation
nameHomo sapiens autophagy related 9A
shRNA_sequence
size2520
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagRFP
resistance markers :
644
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA