This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRS426-TUB1
catalog :
60385
citations: 1
Reference
Sirajuddin M, Rice L, Vale R. Regulation of microtubule motors by tubulin isotypes and post-translational modifications. Nat Cell Biol. 2014;16:335-44 pubmed publisher
product information
Catalog Number :
60385
Product Name :
pRS426-TUB1
article :
doi10.1038/ncb2920
id9095
pubmed_id24633327
bacterial resistance :
Ampicillin
cloning :
backbonepRS426
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Johnson V, Ayaz P, Huddleston P, Rice LM. Design, overexpression, and purification of polymerization-blocked yeast -tubulin mutants. Biochemistry. 2011 Oct 11;50(40):8636-44. doi: 10.1021/bi2005174. Epub 2011 Sep 16.
growth strain :
Expression of yeast alpha tubulin (TUB1) using a GAL promoter
origin :
37
pi :
alt_names
Yeast alpha Tubulin
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterGAL
sequencing_primer_3gggagggcgtgaatgtaagc
sequencing_primer_5aacgtcaaggagaaaaaacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML085C
geneTUB1
id854889
genbank_ids
mutation
nameTUB1
shRNA_sequence
size1500
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
Contains insert obtained from Luke Rice at UT Southwestern
resistance markers :
543
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA