pEGFP-N1-FUS/TLS-FLAGC | Addgene 60362 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP-N1-FUS/TLS-FLAGC

catalog :

60362

citations: 3

Reference
Yang W, Chen X, Zhou Y, Tang X, Sun Y, Dong Y, et al. Investigation of a Fused in Sarcoma Splicing Mutation in a Chinese Amyotrophic Lateral Sclerosis Patient. Can J Neurol Sci. 2022;:1-6 pubmed publisher
Trnka F, Hoffmann C, Wang H, Sansevrino R, Rankovic B, Rost B, et al. Aberrant Phase Separation of FUS Leads to Lysosome Sequestering and Acidification. Front Cell Dev Biol. 2021;9:716919 pubmed publisher
Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B, et al. DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal. Nucleic Acids Res. 2014;42:9047-62 pubmed publisher

product information

Catalog Number :

60362

Product Name :

pEGFP-N1-FUS/TLS-FLAGC

article :

doi	10.1093/nar/gku601
id	9090
pubmed_id	25030905

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-N1-FLAG

backbone_mutation

Insertion of a FLAG tag in front of GFP together with an ATG and a strong kozak sequence.

backbone_origin

backbone_size

4777

promoter

CMV

sequencing_primer_3

GFP-R

sequencing_primer_5

CMV Forward

vector_types

Mammalian Expression

growth strain :

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

origin :

pi :

alt_names

TLS

POMP75

HNRNPP2

cloning

clone_method	Restriction Enzyme
cloning_site_3	MluI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	EGFP-N
sequencing_primer_5	CMVpromoter forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
gene	FUS
id	2521

genbank_ids

NM_004960.3

mutation

name

FUS

shRNA_sequence

size

1586

species

9606

Homo sapiens