pGL4.23 C2_13 | Addgene 60289 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGL4.23 C2_13

catalog :

60289

citations: 1

Reference
Pasquali L, Gaulton K, RodrÃguez SeguÃ S, Mularoni L, Miguel Escalada I, Akerman Ä, et al. Pancreatic islet enhancer clusters enriched in type 2 diabetes risk-associated variants. Nat Genet. 2014;46:136-143 pubmed publisher

product information

Catalog Number :

60289

Product Name :

pGL4.23 C2_13

article :

doi	10.1038/ng.2870
id	9083
pubmed_id	24413736

bacterial resistance :

Ampicillin

cloning :

backbone

pGL4.23-GW

backbone_mutation

backbone_origin

Ferrer Lab

backbone_size

4283

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

chromosome : chr11; start: 133163067 end: 133163569 (genomic position corresponds to the version of the human genome hg18) Fw primer used for cloning: CACCCGAAGGAGTCTGTTGTCAC Rv primer used for cloning: GCCCCTAAAATCAAGGCAATTGAG

growth strain :

This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

origin :

pi :

alt_names

SPATA19

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	RVprimer3 (Promega): CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CT132, SPAS1, spergen1
gene	SPATA19
id	219938

genbank_ids

mutation

name

SPATA19 inactive enhancer

shRNA_sequence

size

species

9606

Homo sapiens