This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHA AKT2 T309A/S474A
catalog :
60128
citations: 2
Reference
Nam H, Jeon S, An H, Yoo J, Lee H, Lee S, et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. elife. 2019;8: pubmed publisher
Das F, Ghosh Choudhury N, Lee D, Gorin Y, Kasinath B, Choudhury G. Akt2 causes TGFβ-induced deptor downregulation facilitating mTOR to drive podocyte hypertrophy and matrix protein expression. PLoS ONE. 2018;13:e0207285 pubmed publisher
product information
Catalog Number :
60128
Product Name :
pHA AKT2 T309A/S474A
article :
doi
id9061
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepLNCX
backbone_mutation
backbone_origin
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth strain :
expresses a mutant AKT2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3LNCX-3
sequencing_primer_5LNCX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
geneAKT2
id208
genbank_ids
mutationSerine 474 and Threonine 309 changed to alanines
nameprotein kinase B
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
37
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA