This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAMPK alpha2 delta312X
catalog :
60127
citations: 4
Reference
Barrett C, Jiang M, O Flaherty B, Dias B, Rainnie D, Young L, et al. Early life exposure to high fructose diet induces metabolic dysregulation associated with sex-specific cognitive impairment in adolescent rats.: Running title: impact of early life fructose diet on adolescent behavior. J Nutr Biochem. 2022;:109220 pubmed publisher
Kim J, Lee H, Cai F, Ko B, Yang C, Lieu E, et al. The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Nat Metab. 2020;2:1401-1412 pubmed publisher
Boon R, Kumar M, Tricot T, Elia I, Ordovas L, Jacobs F, et al. Amino acid levels determine metabolism and CYP450 function of hepatocytes and hepatoma cell lines. Nat Commun. 2020;11:1393 pubmed publisher
Kim J, Hu Z, Cai L, Li K, Choi E, Faubert B, et al. CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells. Nature. 2017;546:168-172 pubmed publisher
product information
Catalog Number :
60127
Product Name :
pAMPK alpha2 delta312X
article :
doi
id9061
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationModification of Dr. Carling's wildtype contruct, Addgene plasmid # 15991
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses constitutively active AMPKalpha2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH rev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAmpk, Ampka2
genePrkaa2
id78975
genbank_ids
mutationTruncated at residue 312
nameAMPK alpha 2
shRNA_sequence
size2000
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagMyc
plasmid copy :
The original plasmid was received from David Carling at the Imperial College School of Medicine in London in 1998
resistance markers :
37
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA