CRY2olig-mCh-CLC | Addgene 60035 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CRY2olig-mCh-CLC

catalog :

60035

citations: 1

Reference
Taslimi A, Vrana J, Chen D, Borinskaya S, Mayer B, Kennedy M, et al. An optimized optogenetic clustering tool for probing protein interaction and function. Nat Commun. 2014;5:4925 pubmed publisher

product information

Catalog Number :

60035

Product Name :

CRY2olig-mCh-CLC

article :

doi	10.1038/ncomms5925
id	9234
pubmed_id	25233328

bacterial resistance :

Kanamycin

cloning :

backbone

pmCherryN1

backbone_mutation

backbone_origin

Clontech

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

*This plasmid contains clathrin light chain A, isoform a, with an A9D mutation relative to NM_001080384 (NP_001073853). This mutation, however, seems to be a natural variant, as it is present in other published sequences for mouse Clta

growth strain :

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

origin :

pi :

alt_names

CRY2

Clathrin Light Chain

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BsrGI
promoter	CMV
sequencing_primer_3	unknown
sequencing_primer_5	mCherry
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
gene	CRY2
id	839529

genbank_ids

mutation

M354I, E490G CRY2, A9D*

name

CRY2-mCh-Clathrin Light Chain

shRNA_sequence

size

2892

species

10090

Mus musculus

3702

Arabidopsis thaliana