This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Human _TFAM_NoMTS_Dimer_pET28a+
catalog :
60013
citations: 2
Reference
Feric M, Demarest T, Tian J, Croteau D, Bohr V, Misteli T. Self-assembly of multi-component mitochondrial nucleoids via phase separation. EMBO J. 2021;40:e107165 pubmed publisher
Ngo H, Lovely G, Phillips R, Chan D. Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation. Nat Commun. 2014;5:3077 pubmed publisher
product information
Catalog Number :
60013
Product Name :
Human _TFAM_NoMTS_Dimer_pET28a+
article :
doi10.1038/ncomms4077
id9040
pubmed_id24435062
bacterial resistance :
Kanamycin
cloning :
backbonepET28a+
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Note that depositor's assembled sequence is WT TFAM; mutations are clear in Addgene quality control sequence.
growth strain :
Expresses TFAM No MTS dimerization mutant in bacterial cells
origin :
37
pi :
alt_names
TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterT7
sequencing_primer_3T7 Terminator
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3
geneTFAM
id7019
genbank_ids
7019
mutationMissing 1st 42 aas (Mitochondrial targeting sequence); Changed K95A, Y99F, E106A, E112A and R116A
nameHuman TFAM No MTS
shRNA_sequence
size618
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis
resistance markers :
665
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA