pLT 653 | Addgene 59996 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLT 653

catalog :

59996

citations: 1

Reference
Timmons L, Luna H, Martinez J, Moore Z, Nagarajan V, Kemege J, et al. Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction. FEBS Lett. 2014;588:3347-51 pubmed publisher

product information

Catalog Number :

59996

Product Name :

pLT 653

article :

doi	10.1016/j.febslet.2014.07.023
id	9030
pubmed_id	25066299

bacterial resistance :

Ampicillin

cloning :

backbone

L4440

backbone_mutation

backbone_origin

Andrew Fire

backbone_size

2790

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325 330.)

growth strain :

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

growth temp :

Utilize HT115(DE3) cells for induction of dsRNA

origin :

pi :

alt_names

B0365.7

cloning

entrez_gene

aliases	CELE_B0365.7
gene	dhc-3
id	179798

genbank_ids

NM_073871

mutation

partial genomic

name

dhc-3

shRNA_sequence

size

465

species

6239

Caenorhabditis elegans