This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJK_pro6_amiE
catalog :
59844
citations: 1
Reference
Bienick M, Young K, Klesmith J, Detwiler E, Tomek K, Whitehead T. The interrelationship between promoter strength, gene expression, and growth rate. PLoS ONE. 2014;9:e109105 pubmed publisher
product information
Catalog Number :
59844
Product Name :
pJK_pro6_amiE
article :
doi10.1371/journal.pone.0109105
id9306
pubmed_id25286161
bacterial resistance :
Ampicillin
cloning :
backbonepET-29b(+)
backbone_mutationDeletion of lacI, lacO, and T7 promoter by cloning proB in-between BglI and XbaI. Removal of the kanamycin resistance gene and insertion of the beta-lactamase (AmpR) gene from pET-22b.
backbone_originNovagen
backbone_size3582
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Constitutively expresses AmiE with pro6 promoter
origin :
37
pi :
alt_names
amiE
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BglI
promoterpro6
sequencing_primer_3T7 Term
sequencing_primer_5GGTGATTCATTCTGCTAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPA3366
geneamiE
id877816
genbank_ids
mutation
nameALIPHATIC AMIDASE
shRNA_sequence
size1024
species
32644
Other
tags
locationC terminal on backbone
tag6x-His
resistance markers :
2077
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA