pSA101 | Addgene 59787 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSA101

catalog :

59787

citations: 1

Reference
Armenti S, Lohmer L, Sherwood D, Nance J. Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. Development. 2014;141:4640-7 pubmed publisher

product information

Catalog Number :

59787

Product Name :

pSA101

article :

doi	10.1242/dev.115048
id	9493
pubmed_id	25377555

bacterial resistance :

Ampicillin

cloning :

backbone	pCFJ150
backbone_mutation	modified to include PmeI restriction site for Gibson cloning
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth strain :

drives ZIF-1 protein in C. elegans neurons

origin :

pi :

3' UTRshRNA_sequencesize705species

6239

Caenorhabditis elegans

tags
Cloningcloning_site_3
cloning_site
_5promotertd>sequencing_pri
mer_3AAACAGTTATGTTTGGTATATTGGG
AATGTsequencing_prime
r_5gtccaattactcttcaacatccctaca
tgctctttctcccsite_3_d
estroyedsite
_5_destroyed
entrez_gene<
/td>genbank_idsable>r>mutationr>nameunc-54

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	CTGAAAATAGGGCTACTGTAGATTTATTTTAAAAGAG
sequencing_primer_5	atcttcagatgggagcagtggactgtc
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

rab-3 promoter

shRNA_sequence

size

species

6239

Caenorhabditis elegans