This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSA107
catalog :
59785
citations: 1
Reference
Armenti S, Lohmer L, Sherwood D, Nance J. Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. Development. 2014;141:4640-7 pubmed publisher
product information
Catalog Number :
59785
Product Name :
pSA107
article :
doi10.1242/dev.115048
id9493
pubmed_id25377555
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ150
backbone_mutationModified to include PmeI restriction site at Gibson cloning site
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
drives ZIF-1 protein in C. elegans ubiquitously
origin :
37
pi :
promotershRNA_sequencesizespecies
6239
Caenorhabditis elegans
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3TTATTGTTGAATATTTATCGTGCACAAATTTTCGGCAG
sequencing_primer_5atgagtgagtgttccgcgagtac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F59B2.6
genezif-1
id176274
genbank_ids
mutation
namezif-1 coding sequence
shRNA_sequence
size
species
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3AAACAGTTATGTTTGGTATATTGGGAATG
sequencing_primer_5gtccaattactcttcaacatccc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameunc-54 3' UTR
shRNA_sequence
size
species
tags

Cloningcloning_site_3
cloning_site
_5promotertd>sequencing_pri
mer_3TTCGCCTGAAAAAAAAAATGAATAA
AAGTATTAAAATTACsequen
cing_primer_5ggtcgagctgaatacac
gtgcacaagsite_3_destr
oyedsite_5_d
estroyed>entrez_genee>d>
aliases
geneNEWENT
RY
id286789td>

genbank_ids<
/tr>mutation
namecdc-42
alt_names
cloning
clone_methodGibson
resistance markers :
2114
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA