This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-Flag-H3.3-polyA
catalog :
59780
citations: 1
Reference
Inoue A, Zhang Y. Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes. Nat Struct Mol Biol. 2014;21:609-16 pubmed publisher
product information
Catalog Number :
59780
Product Name :
pcDNA-Flag-H3.3-polyA
article :
doi10.1038/nsmb.2839
id8958
pubmed_id24908396
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-polyA
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV and T7
sequencing_primer_3CCAAACTTTTATTTGTGCACAT
sequencing_primer_5CACTGCTTACTGGCTTATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRYLIB2, H3-3A, H3.3B, H3F3B
geneH3-3B
id3021
genbank_ids
mutation
nameH3F3B
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
2065
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA