This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tol2-elavl3-GCaMP6s
catalog :
59531
citations: 7
Reference
Yang Y, Briant L, Raab C, Mullapudi S, Maischein H, Kawakami K, et al. Innervation modulates the functional connectivity between pancreatic endocrine cells. elife. 2022;11: pubmed publisher
Pereida Jaramillo E, Gómez González G, Espino Saldaña A, Martinez Torres A. Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development. Int J Mol Sci. 2021;22: pubmed publisher
Hong J, Lee J, Sohn K, Lee K, Lee S, Lee J, et al. IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish. Commun Biol. 2021;4:1405 pubmed publisher
Mruk K, Ciepla P, Piza P, Alnaqib M, Chen J. Targeted cell ablation in zebrafish using optogenetic transcriptional control. Development. 2020;147: pubmed publisher
Müllenbroich M, Turrini L, Silvestri L, Alterini T, Gheisari A, Vanzi F, et al. Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy. Front Cell Neurosci. 2018;12:315 pubmed publisher
Turrini L, Fornetto C, Marchetto G, Müllenbroich M, Tiso N, Vettori A, et al. Optical mapping of neuronal activity during seizures in zebrafish. Sci Rep. 2017;7:3025 pubmed publisher
Vladimirov N, Mu Y, Kawashima T, Bennett D, Yang C, Looger L, et al. Light-sheet functional imaging in fictively behaving zebrafish. Nat Methods. 2014;11:883-4 pubmed publisher
product information
Catalog Number :
59531
Product Name :
Tol2-elavl3-GCaMP6s
article :
doi10.1038/nmeth.3040
id8907
pubmed_id25068735
bacterial resistance :
Ampicillin
cloning :
backboneMiniTol2
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence.
growth strain :
Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone
origin :
37
pi :
alt_names
HuC
cloning
clone_methodRestriction Enzyme
cloning_site_3Age1
cloning_site_5Xho1
promoter
sequencing_primer_3tttaacctctcagcgagaatgc
sequencing_primer_5TGATCTGCAAAAGACGTGAATATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHuC, elrc, id:ibd1248, wu:fb77b03, zHuC
geneelavl3
id30732
genbank_ids
mutation
nameelavl3 promoter
shRNA_sequence
size8700
species
7955
Danio rerio
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Age1
cloning_site_5age1
promoter
sequencing_primer_3ATGGGACAATAACAACCAAGGAG
sequencing_primer_5tttaacctctcagcgagaatgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameGCaMP6s
shRNA_sequence
size
species
100
Synthetic
tags
plasmid copy :
MiniTol2National Institute of Genetics Japan GCaMP6s is from HHMI-Janelia elavl3 promoter is from Brain Science Institute, RIKEN, Japan
resistance markers :
2054
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA