This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tol2-elavl3-H2B-GCaMP6s
catalog :
59530
citations: 5
Reference
Kolb J, Tsata V, John N, Kim K, M xf6 ckel C, Rosso G, et al. Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment. Nat Commun. 2023;14:6814 pubmed publisher
Zarowny L, Aggarwal A, Rutten V, Kolb I, Patel R, Huang H, et al. Bright and High-Performance Genetically Encoded Ca2+ Indicator Based on mNeonGreen Fluorescent Protein. ACS Sens. 2020;5:1959-1968 pubmed publisher
Müllenbroich M, Turrini L, Silvestri L, Alterini T, Gheisari A, Vanzi F, et al. Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy. Front Cell Neurosci. 2018;12:315 pubmed publisher
Garcia M, Karlstaedt A, Chen J, Amione Guerra J, Youker K, Taegtmeyer H, et al. Functionally redundant control of cardiac hypertrophic signaling by inositol 1,4,5-trisphosphate receptors. J Mol Cell Cardiol. 2017;112:95-103 pubmed publisher
Freeman J, Vladimirov N, Kawashima T, Mu Y, Sofroniew N, Bennett D, et al. Mapping brain activity at scale with cluster computing. Nat Methods. 2014;11:941-50 pubmed publisher
product information
Catalog Number :
59530
Product Name :
Tol2-elavl3-H2B-GCaMP6s
article :
doi10.1038/nmeth.3041
id8905
pubmed_id25068736
bacterial resistance :
Ampicillin
cloning :
backboneMiniTol2
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Transposable element
growth notes :
Addgene NGS QC analysis identified multiple variants in the HuC promoter sequence.
growth strain :
Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone
origin :
37
pi :
alt_names
HuC
cloning
clone_methodRestriction Enzyme
cloning_site_3Age1
cloning_site_5Xho1
promoter
sequencing_primer_3tttaacctctcagcgagaatgc
sequencing_primer_5TGATCTGCAAAAGACGTGAATATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHuC, elrc, id:ibd1248, wu:fb77b03, zHuC
geneelavl3
id30732
genbank_ids
mutation
nameelavl3 promoter
shRNA_sequence
size8700
species
7955
Danio rerio
tags
alt_names
Histone
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5tttaacctctcagcgagaatgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameH2B
shRNA_sequence
size378
species
9606
Homo sapiens
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CATCGATGGTACAGTAAAACGACGG
sequencing_primer_5ACTCCAGCAGGACCATGTGAT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameGCaMP6s
shRNA_sequence
size1353
species
100
Synthetic
tags
plasmid copy :
GCaMP6s is from HHMI-Janelia Farm. There might be a patent. MiniTol2 backbone is from National Institute of Genetics Japan. Zebrafish elavl3 promoter is originally came from Brain Science Institute, RIKEN, Japan
resistance markers :
2054
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA