RP185 pUAST EGFP:Or47b | Addgene 59525 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

RP185 pUAST EGFP:Or47b

catalog :

59525

citations: 1

Reference
Benton R, Sachse S, Michnick S, Vosshall L. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol. 2006;4:e20 pubmed

product information

Catalog Number :

59525

Product Name :

RP185 pUAST EGFP:Or47b

article :

doi	10.1371/journal.pbio.0040020
id	8333
pubmed_id	16402857

bacterial resistance :

Ampicillin

cloning :

backbone

pUAST

backbone_mutation

backbone_origin

backbone_size

8904

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Note: Compared to the NCBI genomic reference sequence for Drosophila melanogaster Or47b , Addgene's quality control sequencing finds about ten mismatches in the insert ORF, including I245M and G363D amino acid residue substitutions. The depositing laboratory is aware of these differences, and states that they are polymorphisms that are not expected to affect protein function. The coding changes are not in highly conserved domains in the protein.

growth strain :

N-terminal fusion of GFP to Drosophila melanogaster Or47b

origin :

pi :

alt_names

Or47b

Odorant receptor 47b

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
sequencing_primer_5	Pry1 (5'-ccttagcatgtccgtggggtttgaat-3'); EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	Dmel_CG13206, 47E.2, 47b, CG13206, DOR25, DOR47E.2, Dmel CG13206, OR 47b, OR47b, Or47E.2
gene	Or47b
id	36212

genbank_ids

mutation

name

EGFP:Or47b

shRNA_sequence

size

2100

species

7227

Drosophila melanogaster