This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHRIG-S6K
catalog :
59446
citations: 1
Reference
Xie R, Wang P, Cheng M, Sapolsky R, Ji X, Zhao H. Mammalian target of rapamycin cell signaling pathway contributes to the protective effects of ischemic postconditioning against stroke. Stroke. 2014;45:2769-76 pubmed publisher
product information
Catalog Number :
59446
Product Name :
pHRIG-S6K
article :
doi10.1161/STROKEAHA.114.005406
id8893
pubmed_id25013017
bacterial resistance :
Ampicillin
cloning :
backbonepHR-IG (pHR tripCMV-IRES-eGFP)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The S6K1 gene was cloned from Addgene plasmid # 26610, pcDNA-Myc S6K1. It starts at aa24 compared to GenBank reference sequence NP_114191.1 and contains an additional 7 amino acids after the end of S6K1 gene in this reference sequence and plasmid 26610.
growth strain :
lentiviral expression of S6K and EGFP
origin :
37
pi :
alt_names
Rps6kb1
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NotI
promoterCMV
sequencing_primer_3pCDH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesp70 S6K-alpha
geneRps6kb1
id83840
genbank_ids
mutationS6K starts at aa24 and contains 7 extra amino acids at end of S6K
nameS6K
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagIRES-EGFP
resistance markers :
2051
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA