This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBMN-YFP-Parkin
catalog :
59416
citations: 8
Reference
Rai P, Janardhan K, Meacham J, Madenspacher J, Lin W, Karmaus P, et al. IRGM1 links mitochondrial quality control to autoimmunity. Nat Immunol. 2021;22:312-321 pubmed publisher
Calvelli H, Krigman J, Onishi M, Narendra D, Sun N, Okamoto K. Detection of mitophagy in mammalian cells, mice, and yeast. Methods Cell Biol. 2020;155:557-579 pubmed publisher
Liang J, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, et al. A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Cell. 2020;180:1160-1177.e20 pubmed publisher
Wang C. A Sensitive and Quantitative mKeima Assay for Mitophagy via FACS. Curr Protoc Cell Biol. 2020;86:e99 pubmed publisher
Sarraf S, Sideris D, Giagtzoglou N, Ni L, Kankel M, Sen A, et al. PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Cell Rep. 2019;29:225-235.e5 pubmed publisher
Lindqvist L, Frank D, McArthur K, Dite T, Lazarou M, Oakhill J, et al. Autophagy induced during apoptosis degrades mitochondria and inhibits type I interferon secretion. Cell Death Differ. 2018;25:782-794 pubmed publisher
Fogel A, Martin S, Hasson S. Application of Imaging-Based Assays in Microplate Formats for High-Content Screening. Methods Mol Biol. 2016;1439:273-304 pubmed publisher
Yamano K, Fogel A, Wang C, van der Bliek A, Youle R. Mitochondrial Rab GAPs govern autophagosome biogenesis during mitophagy. elife. 2014;3:e01612 pubmed publisher
product information
Catalog Number :
59416
Product Name :
pBMN-YFP-Parkin
article :
doi
id8877
pubmed_id24569479
bacterial resistance :
Ampicillin
cloning :
backbonepBMN-Z-LacZ
backbone_mutationLacZ is removed during cloning.
backbone_origin
backbone_size4938
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
to make YFP-Parkin stable cell line with retrovirus
origin :
37
pi :
alt_names
Park2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5CCCTCAAAGTAGACGGCATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562
mutation
nameYFP-Parkin
shRNA_sequence
size2142
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEYFP
resistance markers :
604
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA