This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Lenti.pFOXA2.5'.GFP
catalog :
59407
citations: 1
Reference
Aguila J, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, et al. Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells. Stem Cells Transl Med. 2014;3:1032-42 pubmed publisher
product information
Catalog Number :
59407
Product Name :
Lenti.pFOXA2.5'.GFP
article :
doi10.5966/sctm.2014-0011
id8871
pubmed_id25024431
bacterial resistance :
Ampicillin
cloning :
backbonepRRL.cPPT.PGK-GFP.WPRE.Sin-18
backbone_mutationreplaced pPGK promoter in pRRL.cPPT.PGK-GFP.WPRE.Sin-18 with approx. 1350 bp human 5' FOXA2 promoter region using 5' XhoI and 3' AgeI restriction sites. The promoter contains 396 bp upstream of Exon1, untranslated Exon1, and Intron 1 (Ref. Navas MA et al., 2000, Human Heredity 50:370-81). Note: only the cloning sites have been sequenced.
backbone_originD. Trono, EPFL, Lausanne, CH
backbone_size7272
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development
origin :
37
pi :
alt_names
Forkhead box A2 promoter
TCF-3B promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5XhoI
promoterFOXA2
sequencing_primer_3cagcttgccgtaggtggcatc
sequencing_primer_5ggtacagtgcaggggaaag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHNF-3-beta, HNF3B, TCF3B
geneFOXA2
id3170
genbank_ids
mutation
nameapprox. 1350 bp FOXA2 promoter, eGFP
shRNA_sequence
size1350
species
9606
Homo sapiens
tags
resistance markers :
2048
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA