This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNICE HA-mSYD1B
catalog :
59362
citations: 2
Reference
Shi G, Song C, Torres Robles J, Salichos L, Lou H, Lam T, et al. Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactors. Sci Signal. 2023;16:eabm5518 pubmed publisher
Wentzel C, Sommer J, Nair R, Stiefvater A, Sibarita J, Scheiffele P. mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking. Neuron. 2013;78:1012-23 pubmed publisher
product information
Catalog Number :
59362
Product Name :
pNICE HA-mSYD1B
article :
doi10.1016/j.neuron.2013.05.010
id8849
pubmed_id23791195
bacterial resistance :
Kanamycin
cloning :
backbonepNICE-HA-N
backbone_mutation
backbone_origin
backbone_size3984
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mSYD1B can be released without HA-tag (by XhoI / BamHI) or with HA-tag (e.g. by AgeI / BamHI).
growth strain :
Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)
origin :
37
pi :
alt_names
Syde2
synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
mammalian SYD1B
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3pNICE_rev_seq (5'-AAGCAAGTAAAACCTCTACAAATG-3'); SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC430017H16Rik
geneSyde2
id214804
genbank_ids
NM_001166064
mutation
namemSYD1B
shRNA_sequence
size4054
species
10090
Mus musculus
tags
locationN terminal on backbone
tagHA
resistance markers :
444
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA