This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNICE HA-mSYD1A
catalog :
59361
citations: 2
Reference
Han K, Kim Y, Yoon T, Kim H, Bae S, Um J, et al. LAR-RPTPs Directly Interact with Neurexins to Coordinate Bidirectional Assembly of Molecular Machineries. J Neurosci. 2020;40:8438-8462 pubmed publisher
Wentzel C, Sommer J, Nair R, Stiefvater A, Sibarita J, Scheiffele P. mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking. Neuron. 2013;78:1012-23 pubmed publisher
product information
Catalog Number :
59361
Product Name :
pNICE HA-mSYD1A
article :
doi10.1016/j.neuron.2013.05.010
id8849
pubmed_id23791195
bacterial resistance :
Kanamycin
cloning :
backbonepNICE-HA-N
backbone_mutation
backbone_origin
backbone_size4015
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mSYD1A can be released without HA-tag (by SalI / BamHI) or with HA-tag (e.g. by EcoRI / BamHI).
growth strain :
Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)
origin :
37
pi :
alt_names
Syde1
synapse defective 1, Rho GTPase, homolog 1 (C. elegans)
mammalian SYD1A
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SalI
promoterCMV
sequencing_primer_3pNICE_rev_seq (5'-AAGCAAGTAAAACCTCTACAAATG-3'); SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliases1200008N06Rik, BB043000, mSYD1A
geneSyde1
id71709
genbank_ids
NM_027875
mutation
namemSYD1A
shRNA_sequence
size2214
species
10090
Mus musculus
tags
locationN terminal on backbone
tagHA
resistance markers :
444
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA