pLenLox_U6 Nlgn3 | Addgene 59359 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenLox_U6 Nlgn3

catalog :

59359

citations: 1

Reference
Chih B, Engelman H, Scheiffele P. Control of excitatory and inhibitory synapse formation by neuroligins. Science. 2005;307:1324-8 pubmed

product information

Catalog Number :

59359

Product Name :

pLenLox_U6 Nlgn3

article :

doi	10.1126/science.1107470
id	8848
pubmed_id	15681343

bacterial resistance :

Ampicillin

cloning :

backbone

pLL3.7

backbone_mutation

GFP expression is driven by CAG-promoter (instead of CMV-promoter)

backbone_origin

backbone_size

8777

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Suitable for knockdown of rat Nlgn3.

growth strain :

Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

growth temp :

Growth in standard bacterial strains (e.g. DH5alpha) at 37 C is possible, however, growth in SURE cells (37 C) or Stbl3 cells (30 C) is recommended to reduce the risk of recombination.

origin :

pi :

alt_names

Nlgn3

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HpaI
promoter	mouse U6
sequencing_primer_3
sequencing_primer_5	mU6-F (5'-CAGCACAAAAGGAAACTCACC-3'); mU6-F2 (5'-ACAGACTTGTGGGAGAAGC-3')
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases
gene	Nlgn3
id	171297

genbank_ids

NM_134336

mutation

name

Neuroligin 3 shRNA (rat)

shRNA_sequence

size

species

10116

Rattus norvegicus