This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenLox_U6 Nlgn2
catalog :
59358
citations: 2
Reference
Niederkorn M, Hueneman K, Choi K, Varney M, Romano L, Pujato M, et al. TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis. Cell Rep. 2020;30:2776-2790.e6 pubmed publisher
Chih B, Engelman H, Scheiffele P. Control of excitatory and inhibitory synapse formation by neuroligins. Science. 2005;307:1324-8 pubmed
product information
Catalog Number :
59358
Product Name :
pLenLox_U6 Nlgn2
article :
doi10.1126/science.1107470
id8848
pubmed_id15681343
bacterial resistance :
Ampicillin
cloning :
backbonepLL3.7
backbone_mutationGFP expression is driven by CAG-promoter (instead of CMV-promoter)
backbone_origin
backbone_size8777
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Suitable for knockdown of rat and mouse Nlgn2.
growth strain :
Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
growth temp :
Growth in standard bacterial strains (e.g. DH5alpha) at 37 C is possible, however, growth in SURE cells (37 C) or Stbl3 cells (30 C) is recommended to reduce the risk of recombination.
origin :
30
pi :
alt_names
Nlgn2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HpaI
promotermouse U6
sequencing_primer_3
sequencing_primer_5mU6-F (5'-CAGCACAAAAGGAAACTCACC-3'); mU6-F2 (5'-ACAGACTTGTGGGAGAAGC-3')
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneNlgn2
id117096
aliasesNL2, NLG2
geneNlgn2
id216856
genbank_ids
NM_053992
NM_198862
mutation
nameNeuroligin 2 shRNA (rat and mouse)
shRNA_sequence
size54
species
10090
Mus musculus
10116
Rattus norvegicus
tags
alt_names
enhanced green fluorescent protein
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3
sequencing_primer_5pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameeGFP
shRNA_sequence
size720
species
tags
resistance markers :
444
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA