pET-28a-GFP-Patronin CC 868 1457 | Addgene 59053 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-28a-GFP-Patronin CC 868 1457

catalog :

59053

citations: 2

Reference
Kawano D, Pinter K, Chlebowski M, Petralia R, Wang Y, Nechiporuk A, et al. NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals. iScience. 2022;25:105072 pubmed publisher
Hendershott M, Vale R. Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin. Proc Natl Acad Sci U S A. 2014;111:5860-5 pubmed publisher

product information

Catalog Number :

59053

Product Name :

pET-28a-GFP-Patronin CC 868 1457

article :

doi	10.1073/pnas.1404133111
id	8817
pubmed_id	24706919

bacterial resistance :

Kanamycin

cloning :

backbone

pET28a

backbone_mutation

vector contains a His tag, Strep tag and sfGFP; a TEV site can be used to cleave off all these tags to get a native construct

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

for E. coli expression of Patronin CC truncation

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TGTAAAACGACGGCCAGT
sequencing_primer_5	GGAATTGTGAGCGGATAACAATT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG33130, BcDNA:LD17191, CG18459, CG18460, CG18462, CG30102, CG33130, CG6516, Dmel CG33130, Ssp4, l(2)k07433, ssp4
gene	Patronin
id	36978

genbank_ids

mutation

name

Patronin

shRNA_sequence

size

species

7227

Drosophila melanogaster