This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRLsin-SV40 T antigen-IRES-mCherry
catalog :
58993
citations: 7
Reference
Tuano N, Beesley J, Manning M, Shi W, Perlaza Jiménez L, Malaver Ortega L, et al. CRISPR screens identify gene targets at breast cancer risk loci. Genome Biol. 2023;24:59 pubmed publisher
Turkalo T, Maffia A, Schabort J, Regalado S, Bhakta M, Blanchette M, et al. A non-genetic switch triggers alternative telomere lengthening and cellular immortalization in ATRX deficient cells. Nat Commun. 2023;14:939 pubmed publisher
Krappitz M, Bhardwaj R, Dong K, Staudner T, Yilmaz D, Pioppini C, et al. XBP1 Activation Reduces Severity of Polycystic Kidney Disease due to a Nontruncating Polycystin-1 Mutation in Mice. J Am Soc Nephrol. 2022;: pubmed publisher
Malaver Ortega L, Rosenbluh J. Immortalised Cas9-expressing Cell lines for Gene interrogation. Methods Mol Biol. 2022;2495:91-97 pubmed publisher
Ballester M, Bolonio M, Santamaría R, Castell J, Ribes Koninckx C, Bort R. Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector. Stem Cell Res Ther. 2019;10:317 pubmed publisher
Bernal A, Moltó Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells. Oncotarget. 2018;9:27151-27170 pubmed publisher
Holczbauer A, Factor V, Andersen J, Marquardt J, Kleiner D, Raggi C, et al. Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types. Gastroenterology. 2013;145:221-231 pubmed publisher
product information
Catalog Number :
58993
Product Name :
pRRLsin-SV40 T antigen-IRES-mCherry
article :
doi10.1053/j.gastro.2013.03.013
id8795
pubmed_id23523670
bacterial resistance :
Ampicillin
cloning :
backbonepRRLsin.cPPT.PGK/GFP.WPRE
backbone_mutationNotI site at nt 924 was eliminated
backbone_origin
backbone_size6110
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SalI
promoter
sequencing_primer_3pCAG-F
sequencing_primer_5none
site_3_destroyed
site_5_destroyed1
entrez_gene
genbank_ids
mutationEcoRV site in MCS converted to BamHI site
nameCMV enhancer-chicken beta actin promoter
shRNA_sequence
size1730
species
32644
Other
tags
alt_names
SV40gp6
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterCMV enhancer-chicken beta actin
sequencing_primer_3IRES-R
sequencing_primer_5pCAG-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameSV40 large T antigen
shRNA_sequence
size2200
species
32644
Other
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoterCMV enhancer-chicken beta actin
sequencing_primer_3mCherry-F
sequencing_primer_5IRES-R
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameIRES-mCherry red fluorescent protein
shRNA_sequence
size1360
species
100
Synthetic
tags
plasmid copy :
The mCherry sequence was obtained from Dr. Roger Tsien. Other genes were obtained from plasmids purchased from Addgene
resistance markers :
2042
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA