This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
T7-VEE-OKS-iG
catalog :
58974
citations: 4
Reference
Kim M, Hwang S, Yoon J, Jeong Y, Kim E, Hyun S. Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs. Animals (Basel). 2020;10: pubmed publisher
Marczenke M, Piccini I, Mengarelli I, Fell J, Röpke A, Seebohm G, et al. Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells. Front Physiol. 2017;8:469 pubmed publisher
Marczenke M, Fell J, Piccini I, Röpke A, Seebohm G, Greber B. Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation. Stem Cell Res. 2017;21:26-28 pubmed publisher
Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, et al. Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013;13:246-54 pubmed publisher
product information
Catalog Number :
58974
Product Name :
T7-VEE-OKS-iG
article :
doi10.1016/j.stem.2013.06.001
id8771
pubmed_id23910086
bacterial resistance :
Ampicillin
cloning :
backboneT7-VEE-IRES-puro (Addgene plasmid 58970)
backbone_mutation
backbone_originDowdy lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Venezuelan equine encephalitis (VEE) virus RNA replicon
growth notes :
Summary of features: 1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter) 7592-8671: human Oct4 8678-8731: T2A peptide sequence 8738-10147: human Klf4 10154-10213: E2A peptide sequence 10223-11176: human Sox2 11195-11805: IRES sequence 11818-13680: human Glis1 13689-14300: IRES sequence 14301-14900: Puromycin resistance gene 14907-15034: VEE 3 UTR and poly A tail 15203-16063: Ampicillin resistance gene 16843-16860: T7 promoter
growth strain :
RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOct4
shRNA_sequence
size
species
9606
Homo sapiens
tags
alt_names
Kruppel-like factor 4
cloning
entrez_gene
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
mutation
nameKlf4
shRNA_sequence
size
species
9606
Homo sapiens
tags
alt_names
SRY (sex determining region Y)-box 2
cloning
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
mutation
nameSox2
shRNA_sequence
size
species
9606
Homo sapiens
tags
alt_names
GLIS family zinc finger 1
cloning
entrez_gene
aliases
geneGLIS1
id148979
genbank_ids
mutation
nameGLIS
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
1187
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA